Figure 2.

Percentage of read coverage per detected Phyla (a) and Arthropoda Order (b), and taxa richness (≥98% identity) per Arthropoda Order (c), retrieved through metabarcoding of DNA extracted from the 96% ethanol used to preserve five unprocessed samples of freshwater macroinvertebrates. Green bars refer to macroinvertebrate taxa targeted by the Water Framework Directive, and red bars refer to nontarget taxa. NI, not identified [Colour figure can be viewed at http://www.wileyonlinelibrary.com]