Figure 6.

Altered gene expression patterns in the Hox13dKO autopod. Dorsal view of gene expression detected by in situ hybridization in E11.25–11.5 (a, c, e, g, i) wild‐type and (b, d, f, h, j) Hox13dKO forelimb buds. The expression of (a and b) Bmp2, (c and d) Stmn2 and (e and f) Sulf1 in the autopodal mesenchyme was reduced or undetectable in the Hox13dKO embryos. In contrast, (g and h) Shox2 and (i and j) Tshz2 showed ectopic expression in the Hox13dKO autopod. Since Hoxa13 KO mice were generated by inserting Neo sequence in Hoxa13 locus, Neo expression mimics Hoxa13 expression. In order to define the autopod as the region expressing Hoxa13 in the Hox13dKO mice, contralateral limb bud of each specimen was hybridized with Neo probe (results not shown). Scale bar indicates 500 μm