Table 2.
Survival and mutation frequencies in the M2 generation
| Seed condition | Dose (Gy) | LET (keV/um) | Num. of M1 lines harvested | Survival rate (%) | Fertility (%) | Mutation rate (%) | Number of lines with CDMs | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Albino | Palegreen | Yellow | Virescent | Stripe | |||||||
| Dry | 0 | — | 98 | 98 | 100 | 0 | 0 | 0 | 0 | 0 | 0 |
| Dry | 75 | 30 | 95 | 95 | 100 | 6.3 | 3 | 3 | 0 | 0 | 0 |
| Dry | 100 | 30 | 97 | 97 | 100 | 2.1 | 1 | 1 | 0 | 0 | 0 |
| Dry | 150 | 30 | 243 | 81 | 93.7 | 9.9 | 10 | 7 | 3 | 2 | 2 |
| Dry | 175 | 30 | 225 | 75 | 80.3 | 15.6 | 15 | 8 | 2 | 4 | 6 |
| Dry | 200 | 30 | 97 | 97 | 65.0 | 8.3 | 4 | 3 | 0 | 1 | 0 |
| Wet | 15 | 50 | 226 | 75 | n.d. | 6.6 | 6 | 2 | 4 | 0 | 3 |
Survival, which is the percentage of the M1 lines grown from the irradiated seeds in a paddy field, was comparable with that of the non‐irradiated Nipponbare control up to 100 Gy irradiation (95–97% survival compared with the non‐irradiated control). Significant decline in survival was observed for 150 Gy (81%) and 175 Gy (75%) irradiation of dry seeds. n.d., not determined.