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. 2019 Jan 18;97(6):1089–1104. doi: 10.1111/tpj.14181

Figure 4.

Figure 4

Confirmation of qSE3 candidate gene. (a) Comparison of the nucleotide sequence in the open reading frames (ORFs) of OsHAK21 among Nipponbare, Jiucaiqing and IR26. Black boxes indicate exons, and white box shows the 3′ untranslated region. Solid lines represent introns. Nucleotide variation presented below the horizontal line, and amino acid listed in brackets. (b) Model of OsHAK21 topology and location of mutations. The proposed model for OsHAK21 protein is shown with 12 transmembrane domains and along the cytoplasmic C‐terminus. (c) Comparison of the nucleotide sequence in the promoter region of OsHAK21 between Jiucaiqing and IR26. (d) Comparison of the OsHAK21 expression between NIL(qSE3) and IR26 in germinating seeds under normal condions or salinity stress using a qRT‐PCR approach. Gene expression was normalized to that of OsActin gene control. The relative expression levels were represented by fold change relative to the expression level of NIL(qSE3) in aqueous conditions. Each column represents the means ± SD. ** Indicates the significant difference at 1% levels according to Student's t‐test. n.s. Results not significant.