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. 2019 Feb 28;111(4):1025–1038. doi: 10.1111/mmi.14206

Figure 2.

Figure 2

mCherry is the preferred red FP in the periplasm. A. LMC500 carrying DsbAss‐FP‐PBP5 plasmids for the periplasmic expression of the mScarlet FPs and mCh were grown as flask cultures in TY at 37°C and induced with 15 µM IPTG (arrow). Induction did not alter growth rates compared to a control carrying an empty plasmid (EV). B. Fluorescence images of living, fixed and fixed and matured cells showed varying levels of periplasmic fluorescence. Note that the greyscales were set for automatically visualizing any signal. The scale bar represents 2 µm. C. Quantification of fluorescence signals from all samples revealing strong periplasmic signals for mSc‐I and mCherry. The respective number of cells analysed for the living, fixed or fixed and matured samples were: EV 415, 928 and 689, mSc 623, 868 and 1165, mSc‐I 672, 1009 and 952, mSc‐H 618, 569 and 758 and mCh 477, 792 and 1144. The error bars at the mean indicate the 95% confidence interval.