Figure 4.

GM‐CSFRα blockade inhibits inflammation in response to inhaled LPS in a mouse model of acute lung injury. Mice were treated with inhaled PBS or LPS (nebulized 1 mg/mL for 10 min) and 24 hr later BALF total cell counts (A), BALF neutrophils (B) and lung homogenate IL‐1β (C) were assessed 24 hr after LPS challenge. Additionally, mice were treated intranasally with PBS, isotype control, or CAM‐3003 (at doses indicated), or budesonide (3 mg/kg, p.o.) 3 hr prior to LPS exposure as indicated. n = 8 mice per group, except control groups without LPS (n = 4 mice per group). Statistical analysis was performed by 2‐way ANOVA with Bonferroni's post‐test (Significant at ***P < 0.001, **P < 0.01, *P < 0.05 compared to either PBS group, isotype control or LPS group as indicated by bars)