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. Author manuscript; available in PMC: 2020 Aug 6.
Published in final edited form as: Biochemistry. 2019 Jul 26;58(31):3340–3353. doi: 10.1021/acs.biochem.9b00326

Figure 2.

Figure 2.

Solvent−deuterium exchange within DHAP. (a) 1H NMR spectrum of DHAP in 25 mM imidazole/D2O buffer (pH 7.4). (b) 1H NMR spectrum of DHAP (10 mM) after a 21 min incubation with YdjI (2.0 μM). (c) 1H NMR spectrum of DHAP (10 mM) after a 90 min incubation with YdjI (2 μM). (d) 1H NMR spectrum of DHAP (10 mM) after a 4 h incubation with YdjI (2.0 μM) and FBP aldolase (1.0 unit). The reaction is illustrated in Scheme 2.