Table 3.

Steady-State Kinetic Parameters of Selected Substrates with YdjI^a

substrate	kcat (s−1)	kcat/Km (M−1 s−1)
D-fructose-1-Pb	0.042 ± 0.003	7.8 ± 1.0
39b	0.060 ± 0.001	30.5 ± 0.7
40c	<0.010	–
41c	<0.001	–
42b,c	<0.001	–
44	0.011 ± 0.001	26.6 ± 3.5
45	0.860 ± 0.002	(2.1 ± 0.1) × 103
46	0.021 ± 0.012	14.7 ± 1.9
47	0.260 ± 0.084	(4.2 ± 1.6) × 102
50	0.038 ± 0.001	(2.0 ± 0.2) × 102
51	0.089 ± 0.002	(2.0 ± 0.2) × 102

^aErrors were calculated from the standard deviation of the fitting results.

^bCommerically purchased compounds.

^cEstimated on the basis of the lower limit of detection.