Video 3. Normal divisions followed and GLMs in a strain expressing Htb2:mCherry and Myo1:GFP.

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DOI: 10.7554/eLife.50778.010

Cell1: The mother cell undergoes four normal divisions, and on the fifth (at timepoint 7 hr:35 min), it experiences a GLM. The bud neck is clearly maintained until the retrograde transport occurs at 12 hr. Following this event, the bud neck is quickly removed, and is completely gone by 12 hr:15 min. Cell 2: The bud neck is clearly maintained until the retrograde transport occurs at 2 hr:40 min. Following this event, the bud neck is quickly removed, and is completely gone by 2 hr:55 min. The blue arrow points to the mother cell during timepoints where it is experiencing a GLM. Timestamp is Hours:Min.