Figure 6.

Thiostrepton‐mediated anti‐lung CSC effect is associated with an elevated miR‐98 level. A, A panel of microRNAs was examined in CL141 cells, and the miR‐98 level was the most elevated after thiostrepton treatment. B, Comparative quantitative PCR analysis demonstrated that thiostrepton treatment induced elevation of miR‐98 levels in both CL141 parental (P) and sphere (S) cells. *P < .05 (C) Western blotting assays of miR‐98‐mediated effects in both CL141 and CL152 cells. Cancer stemness/EMT markers, including Sox2, Oct‐4A and β‐catenin, were significantly suppressed when miR‐98 mimic was added. In contrast, when treated with miR‐98 inhibitor, increases in Sox2 and Oct‐4A expression were observed in CL141 cells