Figure 5.

Human pancreas and human islet preservation are improved by the use of M101 for pancreas preservation. M101 was injected into the pancreas and in the preservation solution for 3 h. The impact of the molecule was observed both on the pancreatic tissue and islets after isolation. A, HIF1‐α level was assessed by ELISA in pancreatic tissue after 1 h and 3 h of cold ischaemia. AKT activation (phosphorylated protein/total protein ratio), mitochondrial complex 1 activity, and cleaved caspase‐3 levels were assessed in pancreatic tissue by ELISA after 3 h of cold ischaemia. B, Islet isolation was performed, and islets were counted using Islets Equivalents (IEQ) after one night of culture at 37°C. C, The total amount of insulin in islets and (D) insulin secretion of islets under glucose stimulation was quantified by ELISA. Results are expressed as mean ± SEM or median. (M101 treatment n = 3; CTL n = 5)