Figure 4.

The promotive effect of NK‐1R on the NF‐κB was dependent on Akt in vitro. GBC‐SD and NOZ cells were transfected with NF‐κB p65 siRNA for 8 h, the expression of p65 was assessed by western blot (A and B). The transfected GBC‐SD cells were treated with SP, the mRNA levels of IL‐6, IL‐1β, TNF‐α and MMP9 were measured by real‐time RT‐PCR (C). And the protein levels of IL‐6, IL‐1β, TNF‐α and MMP9 were determined by ELISA (D). NOZ cells were transfected with p65 siRNA, with the treatment of SP for indicated times. Real‐time PCR analysis was performed to detect the mRNA expressions of L‐6, IL‐1β, TNF‐α and MMP‐9 (E). ELISA was performed to detect the protein levels of L‐6, IL‐1β, TNF‐α and MMP9 (F). Data are presented as means ± SEM (n = 6). Statistical analysis was performed using one‐way ANOVA coupled with a post hoc test. Significant differences were indicated as *^* P < 0.01 versus Ctrl, ^## P < 0.01 versus SP