Figure 5.

The role of the Akt on NK‐1R‐induced NF‐κB activation. GBC‐SD or NOZ cells were transfected with p65 siRNA, with the treatment of SP. The transfected cell viability was measured using MTT assay (A), The transfected cells were scraped and migration was determined by microscope (B), After pretreated with L703606, GBC‐SD and NOZ cells were stimulated with SP. The Phosphorylation of Akt was determined by western blot (C and D), GBC‐SD and NOZ cells were treated with Akt inhibitor, accompanied by SP treatment. Western blot analyses was performed to investigate nuclear translocation of NF‐κB p65. Histone H3 and GAPDH were used as nuclear and cytoplasmic markers (E and F), Data are presented as means ± SEM (n = 6). Statistical analysis was performed using one‐way ANOVA coupled with a post hoc test. Significant differences were indicated as **P < 0.01 versus Ctrl, ^## P < 0.01 versus SP