Figure 1.

TLR5‐knock‐down lentivirus transfection in 4T1 cell lines and effect on 4T1 cell viability. TLR5‐knock‐down lentivirus transfection efficiency in 4T1 cells (A). The TLR5 mRNA expression between TLR5 ⁺ and TLR5^‐ 4T1 cells was detected by qPCR (n = 3, ^*** P < .01) (B). The TLR5 protein expression between TLR5⁺ and TLR5⁻ 4T1 cells was detected by Western blot (n = 3, ^*** P < .01) (C). CCK8 assay showed TLR5⁻ 4T1 cells had higher proliferation ability than TLR5⁺ 4T1 cells (n = 3, ^* P < .05) (D). Flow cytometry showed there was no difference for cell apoptosis between TLR5⁺ and TLR5⁻ 4T1 cells (n = 3, P > .05) (E).The data are presented as the means ± SD from three independent experiments. The data were analysed by Student's t test. ^* P < .05, ^*** P < .01