Figure 2.

In vitro evaluation of prepared radiolabelled tracers. Representative saturation binding curve and Scatchard plots of ¹²⁵I‐anti‐TLR5 mAb binding to TLR5⁺ 4T1 cells (A) and TLR5‐ 4T1 cells (B). The concentration of the labelled tracer (¹²⁵I‐anti‐TLR5 mAb or ¹²⁵I‐IgG) was kept constantly, and increasing concentrations of unlabelled anti‐TLR5 mAb were used to compete with the ¹²⁵I‐anti‐TLR5 mAb binding (C)