Figure 3.

Whole‐body phosphor‐autoradiography and fluorescence imaging. Representative images were obtained at 24, 48 and 72 h post‐injection of ¹²⁵I‐anti‐TLR5 mAb and showed apparently radioactivities in TLR5⁺ 4T1 tumour (A). Representative images showed no significant radioactivity accumulation TLR5⁺ 4T1 tumour at 24, 48 and 72 h post‐injection of ¹²⁵I‐antiTLR5 mAb in block group (30 min prior to injection of ¹²⁵I‐anti‐TLR5 mAb, 100 μg anti‐TLR5 mAb in 100 μL was injected through the tail vein （B). Representative images were obtained at 24, 48 and 72 h post‐injection of ¹²⁵I‐IgG (C). Representative images of isolated tumours (isolated from model mice in Figure 3A at 72 h) (D). Representative fluorescence image of 4T1 tumour‐bearing mouse model (E).For A, B, C and E, the arrow on the left referred to the TLR5‐ tumour (blue circle), and the right is TLR5⁺ tumour (pink circle). For E, the bottom arrow referred to the TLR5⁺ tumour (without lentivirus transfection) (black circle). The data are from three independent experiments