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. 2019 Sep 29;23(12):8258–8268. doi: 10.1111/jcmm.14698

Figure 4.

Figure 4

ZFAS1 regulated USF1 expression by directly binding miR‐296‐5p as a ceRNA manner and affected tumour growth in vivo. A, The binding motif of miR‐296‐5p on ZFAS1 3′UTR predicted by TargetScan database. B, Relative luciferase activity between miR‐296‐5p and ZFAS1 was pulled up by shifting sequence using pmirGLO‐ZFAS1‐Mut. C, The miR‐296‐5p was significantly down‐regulated in tumour tissues compared with adjacent normal tissues. D, Inhibition of miR‐296‐5p rescued the si‐ZFAS1‐1 transfected RBE and CCLP1 cell vitality. E, The predicted motif and shifted sequences of USF1 3′UTR. F, Relative luciferase activity between miR‐296‐5p and USF1 3′UTR was pulled up by shifting sequence using pmirGLO‐USF1‐Mut. G, Inhibition of miR‐296‐5p rescued the shZFAS1 transfected xenograft tumour growth on volumes and weights in vivo. H, Up‐regulated ZFAS1 or inhibited miR‐296‐5p promoted USF1 expression