Figure 5.

USF1 was up‐regulated in RBE and CCLP1 cells and promoted cell proliferation and metastasis. A, USF1 was up‐regulated in RBE and CCLP1 cells compared with HIBEC. B, Knockdown of USF1 significantly suppressed RBE and CCLP1 cell vitalities evaluated by CCK‐8 assay. C, Knockdown of USF1 suppressed RBE and CCLP1 cell migration ability. D, Knockdown of USF1 promoted RBE and CCLP1 apoptosis detected by Hoechst 33342 staining. E, Knockdown of USF1 suppressed cell EMT progression detected by immunofluorescence of vimentin expression and cell morphology. F, Knockdown of USF1 suppressed Bax and PCNA expression but enhanced E‐cadherin expression