Figure 4.

MiR‐590‐3p targeted TGFBR2 and repressed TGFBR2 expression. (A) The interaction between miR‐590‐3p and TGFBR2 3'UTR. (B) Luciferase activity was determined in cells co‐transfected with different luciferase reporter vectors and miRNAs. (C) The mRNA and (D) protein expression levels of TGFBR2 in cells transfected with miR‐590‐3p mimic or miR‐NC. (E) The mRNA and (F) protein expression levels of TGFBR2 in cells transfected with miR‐590‐3p mimic or miR‐NC. (G) MiR‐590‐3p and (H) TGFBR2 mRNA expression in the clinical samples from normal (n = 30) or OA (n = 30) articular cartilages were analysed by qRT‐PCR. (I) The correlation between PART‐1 and miR‐590‐3p expression level in OA articular cartilages. (J) The correlation between PART‐1 and TGFBR‐2 mRNA expression level in OA articular cartilages. N = 3, significant differences were presented as *P < .05, **P < .01 and ***P < .001