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. 2019 Oct 4;23(12):8233–8245. doi: 10.1111/jcmm.14694

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© 2019 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Macrophage polarization at the site of AA. A, M1 macrophage markers (iNOS) and total macrophage F4/80 were observed using immunofluorescence staining in the tissues collected at the site of AA on day 14. A decrease in iNOS+ cells was observed in both BM‐MSC and MSCs‐CM group comparing with the control medium group. B, The average percentage of iNOS+ cells were normalized with total macrophage F4/80+ cells and statistically analysed. Significantly reduction in iNOS+ cells was observed in the BM‐MSC group and MSCs‐CM group comparing with control medium group. C, M2 macrophage markers (CD206) and total macrophage F4/80 were observed using immunofluorescence staining in the tissues collected at the site of AA on day 14. An increase in CD206+ cells was observed in both BM‐MSC and MSCs‐CM group comparing with the control medium group. D, The average percentage of CD206+ cells were normalized with total macrophage F4/80+ cells and statistically analysed. Significantly increase in CD206+ cells was observed in both BM‐MSC and MSCs‐CM group comparing with the control medium group