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. 2019 Aug 7;317(4):C857–C866. doi: 10.1152/ajpcell.00281.2019

Fig. 6.

Fig. 6.

Cysteinyl leukotriene (CysLT1) receptors are not functionally expressed in human embryonic kidney 293 (HEK293) cells. A: representative traces of acetylcholine (ACh) concentration-response curve (CRC) in HEK-293 cells using Fluo-8 calcium assay. B: ACh CRC in HEK293 cells using Fluo-8 calcium assay (mean ± SE EC50 = 693 ± 0.05 nM, n = 5). C: representative traces of leukotriene D4 (LTD4) CRC in HEK293 cells transiently transfected with CysLT1R using Fluo-8 calcium assay (HEK293 cells alone did not show a response to LTD4). D: LTD4 CRC in HEK293 and HEK293 cells transiently transfected with CysLT1R (mean ± SE EC50 = 46 ± 19 nM in HEK293 + CysLT1R cells; n = 5) using Fluo-8 calcium assay. E: representative traces of HEK293 cells treated with hypotonic (Hypo), isotonic (Iso), or isotonic + 100 nM LTD4 solutions using Ozzy-quenching assay. Solutions are added at time “a” and NaI was added at time “b” (n = 16). F: Ozzy-quenching assay summary data from E. There was no significant difference (n.s.) between isotonic and isotonic + 100 nM LTD4 groups (one-way ANOVA and Kruskal-Wallis post hoc test).