Fig. 5.

Immunocytochemical assessment of sarcomere integrity in enriched cardiomyocytes derived from GS-2 embryonic stem (ES) cells, following exposure to monochromatic light. Enriched GS-2 ES cell-derived functional cardiomyocytes showing constitutive expression of enhanced green fluorescent protein (EGFP; A, E, I, M, and Q) and sarcomere organization evidenced by cardiac troponin I (cTnI) immunostaining (B, F, J, N, and R), counterstained with nuclear stain DAPI (C, G, K, O, and S) before exposure to monochromatic light (A–D), following exposure to blue (E–H), green (I–L), and red (M–P) lights, and after recovery of beating (Q–T). Scale bar: 20 µm.