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. 2019 Jul 31;317(4):C788–C799. doi: 10.1152/ajpcell.00250.2019

Fig. 4.

Fig. 4.

S100A11-receptor for advanced glycation end products (RAGE) signaling pathway regulates gene expression through activation of NF-κB. A: gene expression analysis by quantitative RT-PCR in wild-type bone marrow-derived macrophages (BMDM) treated with 603B-derived extracellular vesicles (EVs) in the presence or absence (NoTx) of the IKKα/β inhibitor TPCA-1 (5 μM) for 6 h (n = 4, from independent BMDM and EV isolations). Target gene expression normalized to 18s. Data represent mean of fold increase over control (untreated BMDM) ± SE. Two-tailed Student’s t-test: *P < 0.05, **P < 0.005. B: representative immunoblot analysis of total and phosphorylated IκBα in total cell lysates from wild-type BMDM incubated with 603B-derived EVs for the indicated time. Actin was used as a loading control. C: representative immunocytochemistry for the NF-κB subunit p65 on wild-type BMDM incubated with 603B-derived EVs for the indicated time, in the presence or absence of TTP448 or TPCA-1 (p65: green; DAPI: blue). Scale bar = 10 μm.