Table 2.

Genetic tools of potential application to achieve inducible connexin and pannexin manipulations of glial cells in vivo.

Cre-expressing lines
Cell-type selectivity (in nervous system)	Construct	Induction method	RRID	Reference
Astrocytes	Aldh1l1-Cre/ERT2 BAC transgene	Tamoxifen	IMSR_JAX:029655	Srinivasan et al. (2016)
Astrocytes, adult neural progenitors cells	Nestin-CreER transgene	Tamoxifen	IMSR_JAX:012906	Burns et al. (2007)
Astrocytes, adult neural progenitors cells	PAC Fgfr3-icreERT2 transgene	Tamoxifen	IMSR_JAX:025809	Rivers et al. (2008)
Oligodendrocytes, Schwann cells, olfactory ensheathing glia, adult neural progenitors	PLP1-Cre/ERT transgene	Tamoxifen	IMSR_JAX:005975	Doerflinger et al. (2003); Guo et al. (2010) and Piantanida et al. (2019)
NG2 cells (oligodendrocyte and astrocyte precursors)	NG2-Cre/ER™ BAC transgene	Tamoxifen	IMSR_JAX:008538	Zhu et al. (2011)
Microglia, mononuclear phagocyte system	Cx3cr1-Cre/ER transgene	Tamoxifen	IMSR_JAX:020940	Yona et al. (2013) and Zhao et al. (2019)
Microglia, mononuclear phagocyte system	Cx3cr1-Cre/ER/YFP transgene	Tamoxifen	IMSR_JAX:021160	Parkhurst et al. (2013)
Microglia, mononuclear phagocyte system	Iba1-tTA transgene	Doxicycline	IMSR_RBRC05769	Tanaka et al. (2012)
Cre-responding lines
Cell-type selectivity (in nervous system)	Construct	Expressed protein/s	RRID	Reference
Astrocytes	Aldh1l1-loxP-EGFP-4XpolyA-loxP-diptheria toxin A (DTA)-polyA BAC transgene	EGFP before recombination, DTA after recombination	IMSR_JAX:026033	Tsai et al. (2012)
Viral vectors
∣rule Cell-type selectivity (in nervous system)	Construct	Expressed protein/s	RRID	Reference
Astrocytes (adult non-neurogenic regions)	AAV(5)-GFAP(2.2)-iCre	Cre recombinase		García-Cáceres et al. (2016)
Astrocytes	AAV-DJ-hALDH1L1-Cre	Cre recombinase		Koh et al. (2017)
Connexin and pannexin floxed lines
Targeted gene	Targeted sequence	Targeted connexins	RRID	Reference
Gja1	Exon 2	Cx43	IMSR_JAX:008039	Liao et al. (2001) and Clasadonte et al. (2017)
Gjb2	Exon 2	Cx26		Cohen-Salmon et al. (2002)
Panx1	Exon 3/4	Px1		Dvoriantchikova et al. (2012) and Poon et al. (2014)