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. 2019 Nov 6;10:2478. doi: 10.3389/fimmu.2019.02478

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Copyright © 2019 Bornhöfft, Rebl, Gallagher, Viergutz, Zlatina, Reid and Galuska.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Siglecs expressed in bovine neutrophils. (A) Cartoon of the Siglecs expressed by bovine neutrophils and their functional intracellular domains. The number of C2-set Ig-like domains was determined by SMART (http://smart.embl-heidelberg.de/smart/set_mode.cgi?NORMAL=1) based on the following NCBI sequences: Siglec-2: XP_024834447.1; Siglec-4: XP_0248341235.1; Siglec-5: XP_005219620.2; Siglec-8: XP_010813599.1; Siglec-14: XP_019834314.1. (B) Pie chart of the percentage Siglec expression in bovine neutrophils. qPCR raw data was normalized against the reference genes. Siglec-2, -4, -5, -8, and -14 were detected by RT-qPCR and the total of the detected Siglecs was set to 100%. (C) Determined copies of Siglecs per ng RNA used during qPCR. X-axis shows the Siglecs expressed by bovine neutrophils; Y-axis shows the log 10 values. Mean values and standard deviations are displayed in the diagrams (Siglec-2, Siglec-5, Siglec-8 I, Siglec-8 II, n = 3 different animals; Siglec-4, Siglec-14, n = 4 different animals). For Siglec-8, values generated with two different primer pairs are displayed.