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. 2019 Nov 12;9:16616. doi: 10.1038/s41598-019-51877-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Kinetics of replication of parental (P0-H3N2) and mouse-adapted (MA-H3N2) A/H3N2 viruses in MDCK and ST6-GalI-MDCK cells. Confluent cells were infected with each of the viruses at a multiplicity of infection (MOI) of 0.0001 PFU/cell. Supernatants were harvested at 12, 24, 36, 48, 72, and 96 h postinfection and titrated by end point titration in 96-well plates. Titers are expressed in log₁₀ TCID50/ml. The dashed horizontal line indicates the lower limit of detection.