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. 2019 Nov 12;9:16616. doi: 10.1038/s41598-019-51877-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lung histopathology on day 6 post-infection in IC and IS C57BL/6 mice intranasally infected with 1 × 10⁴ PFU/mouse of P0-H3N2 and MA-H3N2 viruses. (A) Histological scores of IC and IS mice. (B) Pulmonary histology of IS mice infected with P0-H3N2 (upper left; inflammation score 0), IS mice infected with MA-H3N2 (lower left; inflammation score 7), IC mice infected with P0-H3N2 (upper right; inflammation score 0) and IC mice infected with MA-H3N2 (lower right; inflammation score 11). All photomicrographs are from 4 µm thick sections of formalin-fixed paraffin-embedded lung tissue stained with hematoxylin-eosin scanned with a Nanozoomer 2.0 (Hamamatsu, Japan). Micrometric scale of 500 µm.