Figure 4.

Deletion or expression of dominant negative DRP1 affects total BAX recruitment. Box and whisker plots show the (A) BAX recruitment initiation time (from staurosporine addition) and (B) rate of BAX recruitment. There were no significant differences among all conditions for either the initiation or rate of BAX recruitment (p = 0.75, p = 1.0, respectively, by one-way ANOVA.) (C) BAX recruitment sigmoid curves defined by median optimal parameters for each set of BAX recruitment curves was plotted for each condition (see Fig. S7 for complete set of curves). The median plateau value (Y-max) for BAX recruitment is reduced by 25% in cells lacking DRP1 and 20% in cells expressing the R247E DRP1 dominant-negative mutant, respectively. (D) Box and whisker plot of Y-max values reveal that HeLa wild type cells and HeLa^DRP1−/− cells expressing exogenous WT DRP1 have significantly less BAX recruited than HeLa^DRP1−/− cells and HeLa cells expressing R247E DRP1 (**p < 0.01).