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. 2019 Nov 12;9:16543. doi: 10.1038/s41598-019-53038-z

Figure 7.

Figure 7

Colocalization studies after treatment with the monomers. SDC transfectants (established in K562 cells) were treated with either of the fluorescently labeled α−syn, tau monomers (at a concentration of 5 µM) or Trf (25 µg/ml) for 1 h or 18 h at 37 °C. After incubation, the cells were permeabilized and treated with the respective APC-labeled SDC antibody. Nuclei of cells were stained with DAPI and cellular uptake was then analyzed with CLSM. (a–c) CLSM images of SDC transfectants 1 h after treatment with either of the fluorescently labeled monomers (α−syn, tau) or Trf. Representative images of three independent experiments are shown. Scale bar = 10 μm. (d) Mander’s overlap coefficient (MOC) ± SEM for the overlap of SDCs with either of the fluorescently labeled monomers proteins (α−syn, tau) and Trf was calculated by analyzing 21 cellular images (7 images per sample, experiments performed in triplicate). (e–g) CLSM images of SDC transfectants 18 h after treatment with either of the fluorescently labeled monomers (α−syn, tau) or Trf. SDCs are labeled with the respective APC-labeled SDC antibody. Representative images of three independent experiments are shown. Scale bar = 10 μm. (h) MOC ± SEM for the overlap of SDCs with either of the fluorescently labeled monomers proteins (α−syn, tau) and Trf was calculated by analyzing 21 cellular images (7 images per sample, experiments performed in triplicate).