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. 2019 Nov 12;9:16543. doi: 10.1038/s41598-019-53038-z

Figure 8.

Figure 8

Effect of SDC3 overexpression on α−syn and tau fibrillation and uptake in SH-SY5Y cells. SDC3 overexpressing transfectants created in SH-SY5Y cells (either differentiated [DIF] or undifferentiated [UD]), along with WT SH-SY5Y cells (DIF or UD) were treated with fluorescently labeled (FITC) or unlabeled monomeric α−syn or tau at a concentration of 5 μM at 37 °C for 1 and 18 h. Cells were then processed for uptake and fibrillation studies. (a) Flow cytometry histograms representing α−syn and tau uptake of WT SH-SY5Y cells and SDC3 transfectants 1 and 18 h after treatment with the monomers. UD: undifferentiated; DIF: differentiated SH-SY5Y cells. (b) Fold change in α−syn and tau uptake and fibrillation due to SDC3 overexpression 1 and 18 h after treatment with the monomers. The bars represent mean ± SEM of five independent experiments. Statistical significance vs α−syn or tau treated WT SH-SY5Y cells (standards) was assessed by analysis of variance (ANOVA). *p < 0.05 vs α−syn or tau treated WT SH-SY5Y cells as standards. UD: undifferentiated; DIF: differentiated SH-SY5Y cells. (c,d) Scanning electron microscope visualization of cellular surface of α−syn and tau-treated WT SH-SY5Y cells, SDC3 transfectants. (c) Undifferentiated (UD) and (d) differentiated (DIF) SH-SY5Y cells. (e) Scanning electron microscope visualization of α−syn and tau fibrils formed on SDC3 transfectants. Representative images of three independent experiments are shown. Scale bar = 1 μm. (f) CLSM visualization of ThT labeled, intracellular α−syn and tau fibrils in WT SH-SY5Y cells and SDC3 transfectants 18 h after treatment with α−syn and tau monomers. Representative images of three independent experiments are shown. Scale bar = 10 μm. UD: undifferentiated; DIF: differentiated SH-SY5Y cells.