Fig. 2. Autophagy was activated in A549 lung cancer cells treated with ZBM-H.

A549 cells were treated with ZBM-H for 1 h a–c, 3 h d–f, and 6 h g–i the protein levels of LC3B-II/LC3B-I and SQSTM1 were determined by Western Blot. Rapamycin (Rapa) was used as a positive control. j–l Western Blot analysis of LC3B-II/LC3B-I and SQSTM1 in cells treated with BafA1 (50 nM) and ZBM-H for 6 h, with Rapa (10 μM) as positive control. Data are presented as the mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, n = 3