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. 2019 Nov 12;9:16564. doi: 10.1038/s41598-019-52975-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Competition assay between S. aureus and P. aeruginosa. (A) Competition test on agar plate. S. aureus and P. aeruginosa were grown on BHI for 8 hours at 37 °C. A layer of S. aureus was added on a TSA. After drying, a drop of P. aeruginosa was spotted. The inhibition halo indicates a competition state (SA2597 + PA2596 and SA2599 + PA2596). (B) Competition assay in planktonic culture. S. aureus and P. aeruginosa were mono-cultivated and co-cultivated for 8 hours. Every two hours, bacteria were plated on mannitol salt agar (MSA) and cetrimide to count S. aureus and P. aeruginosa, respectively. The results show one representative experiment from a triplicate. Upper panels, pairs in coexistence. Lower panels, pairs in competition.