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. 2019 Nov 12;9:16564. doi: 10.1038/s41598-019-52975-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confirmation of S. aureus gene expression dysregulation by P. aeruginosa. Twelve clinical SA-PA strain pairs were co-cultivated for 4 hours. RNAs were extracted and RT-qPCR were performed on 15 genes. The results are represented as fold change of expression (gene relative expression in coculture/gene relative expression in monoculture) on a heatmap. Under-expressed genes are indicated in green whereas over-expressed genes are indicated in red. No RNA detection is shown in grey. Pairs were hierarchically clustered by the Euclidean method.