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. 2019 Nov 12;9:16564. doi: 10.1038/s41598-019-52975-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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S. aureus nor gene over-expression requires close contact with P. aeruginosa. S. aureus was deposited onto the bottom of wells. P. aeruginosa was added either with S. aureus (black) or into the insert of transwells (gray). RNAs were extracted and nor gene expression was monitored by RT-qPCR. Dotted lines represent fold change = 2. The results are shown as the mean + standard deviation of three independent experiments on SA30-PA30 pair. Statistical analysis was performed by unpaired t-test (*P < 0.05, **P < 0.01).