Figure 1.

Naturally occurring pyrrolo[2,3‐d]pyrimidine(7‐deazapurine) nucleosides and derivatives efficiently induce human pancreatic cancer cell death. (A) Indicated cell lines were seeded in 96 well plates and treated with indicated compounds at indicated dosage for 48 hours. Cell survival was determined using the MTT assay. Error bars represent standard deviation. (B) Indicated cell lines were seeded in 96 well plates and treated with Sangivamycin at indicated dosage for 48 hours. Cell survival was determined using the MTT assay. Error bars represent standard deviation. (C) Indicated cell lines were seeded in 96 well plates and proliferation was measured after 24, 48 and 72 hours using the CyQUANT Cell Proliferation Assay. Error bars represent standard deviation. (D) Panc1 cells were seeded in 96 well plates and treated with Gemcitabine at indicated dosages either in presence of vehicle or Sangivamycin at EC₂₅ (62.5 nM) for 48 hours. Cell survival was determined using the MTT assay. Error bars represent standard deviation.