TABLE 4.

Comparison of metabolite content of milk between healthy cows and S. agalactiae-infected cows.

Class	Name	KEGG ID	VIPa	p-valueb	FCc	Tendency
Organic Acids	Hydroxypropionic acid	C01013	1.7	0.005	0.427	↓
	Uric acid	C00366	1.7	0.012	0.491	↓
	Phenylpyruvic acid	C00166	1.7	0.014	2.628	↑
	Homogentisic acid: 4-Hydroxyphenylpyruvic acid	C00544: C01179	1.6	0.022	2.923	↑
	Oxoglutaric acid	C00026	1.2	0.025	0.397	↓
	Pyrophosphate	C00013	1.5	0.028	0.281	↓
	Quinic acid	C06746	1.5	0.031	0.497	↓
	Phosphoenolpyruvic acid	C00074	1.4	0.048	0.488	↓
Carbohydrates	Gluconolactone	C00198	1.6	0.017	0.437	↓
	Allose	C01487	1.6	0.018	0.332	↓
	1,5-Anhydrosorbitol	C07326	1.6	0.015	0.334	↓
	Sorbitol	C00794	1.6	0.015	0.533	↓
	L-Arabitol	C00532	1.7	0.019	0.483	↓
	Glucose 1-phosphate	C00103	1.7	0.019	0.162	↓
	D-Mannose	C00159	1.5	0.037	0.353	↓
	Glucose 6-phosphate	C00092	1.4	0.048	0.341	↓
Nucleotides	Uracil: uridine	C00106: C00299	1.7	0.018	0.2	↓
	Xanthine: guanine	C00385: C00242	1.5	0.038	14.29	↑
	Guanine	C00242	1.5	0.045	0.33	↓
	Uridine	C00299	1.7	0.016	4.565	↑
Amino Acids	L-Asparagine	C00152	1.4	0.037	0.493	↓
Alcohols	Glycerol	C00116	1.5	0.043	5.139	↑

^aVIP: variable importance in the projection obtained from the OPLS model with a cut-off of 1.5. ^bp-Values from non-parametric Wilcoxon-Mann-Whitney test. ^cFC: fold change, the ratio of the mean value of the peak area obtained from the S. agalactiae group and the mean value of the peak area obtained from the healthy group. An FC > 1 means that the metabolite is higher in milk from S. agalactiae-infected cows than in milk from healthy cows.