Skip to main content
. 2019 Nov 12;9:16636. doi: 10.1038/s41598-019-53305-z

Figure 5.

Figure 5

ALA activated h/m PXR and inhibited NF-κB-luciferase activity in a hPXR dependent manner. (A) HT-29 cells were co-transfected with CYP3A4-luciferase reporter combined with pRL-TK, and the wild-type hPXR expression construct (pSG5-hPXR) or the wild-type mPXR expression construct (pSG5-mPXR). Cells were incubated with ALA (0–25 μM) and rifampicin or ALA (0–25 μM) and PCN (5 μM) for 24 h. Cell extracts were assayed for luciferase activity. (B) LS174T cells were electroporated with the hPXR siRNA or the control siRNA. Cells were subjected to western blot analysis to determine protein expression (Left panel) or subjected to NF-κB reporter assay as described in Methods (Right panel). The results were presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control cells; #P < 0.05, ###P < 0.001 vs. LPS alone treatment cells.