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. 2019 Nov 12;9:16566. doi: 10.1038/s41598-019-53070-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Multi-dimensional separation workflow. Data shown are taken from this study to represent the value of each step. (A) SEC separation of the partially depolymerized Hp/HS, enoxaparin sodium; (B) chemo-selective reductive amination of one SEC fraction (dp8) with AEAB; (C) IPRP separation of AEAB-labeled oligosaccharide SEC fraction (dp8); IPRP fractions could be either analyzed by (D) online HILIC LC/MS (IPRP fraction RT 35 to 36 min shown); or (E) separated by offline HILIC with fractions collected (IPRP fraction RT 36.8 to 38 min shown); (F) microarray immobilization of HILIC fractions and protein binding affinity assay (IPRP and HILIC fraction identities shown in Table S1).