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De-N-acetylation of GAG. Degree of acetylation (DA) of GAGs (A). DA was determined by an acetate assay (GAG, native polymer; dGAG, de-N-acetylated) (B). In vitro IL-1Ra secretion in PBMC supernatants in presence of GAG. LPS (10 ng/ml) served as external standard and were set to 100% in each biological replicate. Statistical analysis by WMW test in comparison to GAG: ns, non significative; *p < 0.05; ***p < 0.001.
