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. 2019 Nov 6;9:365. doi: 10.3389/fcimb.2019.00365

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Copyright © 2019 Gressler, Heddergott, N'Go, Renga, Oikonomou, Moretti, Coddeville, Gaifem, Silvestre, Romani, Latgé and Fontaine.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chemical analysis and IL-1Ra inducing activity of oligosaccharides generated by acid hydrolysis of dGAG. (A) Summerized MALDI-TOF MS analysis of fractions I-VIII. (DP, degree of polymerization; for detailed MS spectra see Figure S2). (B) IL-1Ra production by dGAG oligosaccharides. dGAG oligosaccharides fractions I-VIII were tested at 25, 5, and 1 μg/ml. dGAG (5 and 1 μg/ml) served as positive control. LPS (10 ng/ml) served as external standard and were set to 100% in each biological replicate. Wilcoxon-Mann-Whitney Test: **p < 0.01; ***p < 0.001 (compared to control, M).