Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 6;9:365. doi: 10.3389/fcimb.2019.00365

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Gressler, Heddergott, N'Go, Renga, Oikonomou, Moretti, Coddeville, Gaifem, Silvestre, Romani, Latgé and Fontaine.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

IL-1Ra stimulation by GAG oligomers is abolished by GAGnase. (A) Fraction F-I and F-II are degraded by GAGnase. Fractions I and II (500 μg/ml) were enzymatically hydrolyzed by GAGnase (2 μg/ml) for 10 or 120 min or were kept in acetate buffer for 120 min (untreated). The degradation was followed by PABA assay to quantify sugar reducing end. (B) Induction of IL1-Ra expression in PBMCs by GAGnase treated fractions. (C) IL-1Ra induction of PBMCs in presence of GAGnase (20 ng/μl). GAGnase neither activate nor inhibit induction. LPS (10 ng/ml) served as reference control and were set to 100% in each biological replicate. bars indicate the SD, WMW Test: ***p < 0.001; ns, not significant (compared to control samples).