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. 2019 Nov 6;9:365. doi: 10.3389/fcimb.2019.00365

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Copyright © 2019 Gressler, Heddergott, N'Go, Renga, Oikonomou, Moretti, Coddeville, Gaifem, Silvestre, Romani, Latgé and Fontaine.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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N-acetylation of de-N-acetylated GAG oligosaccharides and its impact on the IL-1Ra production. (A) Determination of the degree of acetylation (DA) by acetate assay of de-N-acetylated and acetylated fractions I, II and III. GalN and GalNAc served as positive and negative controls. (B) In vitro IL-1Ra production by de-N-acetylated and acetylated GAG fractions I, II, and III (10 and 1 μg/ml). LPS (10 ng/ml) served as positive control. WMW Test: **p < 0.01, ***p < 0.001.