Table 1.
Characteristics of the Enterococcus faecium strains used in this study.
| Strain ID, year of isolation, patient gender, patient age | VAN MIC by E-test | VAN MIC by broth micrcodilution | VAN (Disk diffusion 5 μg) | Growth on BHIA + 6 mg/l VAN | Teicoplanin MIC by E-test | Daptomycin MIC by E-test | Linezolid MIC by E-test |
|---|---|---|---|---|---|---|---|
| E. faecium VanB (NARA-89) | 6 (R) | 4 (S) | R* | + | 0.38 (S) | 1 (S) | 0.75 (S) |
| E. faecium VanB (NARA-388) | 6 (R) | 16 (R) | R* | + | 1.5 (S) | 2 (S) | 0.75 (S) |
| E. faecium VanB (NARA-490) | 32 (R)** | 64 (R) | R* | + | 1 (S) | 1.5 (S) | 1 (S) |
| E. faecium VanB (NARA-491) | 96 (R)** | >64 (R) | R* | + | 1 (S) | 2 (S) | 1 (S) |
| E. faecium VanB (NARA-492) | 4 (S) | 8 (R) | R* | + | 0.75 (S) | 3 (S) | 0.75 (S) |
|
E. faecium (2018, Female, 85 y) HUG |
256 (R) | >64 (R) | R | + | 48 (R) | 3 (S) | 1 (S) |
|
E. faecium (2018, Female, 68 y) HUG |
256 (R) | >64 (R) | R | + | 1.5 (S) | 3 (S) | 1 (S) |
|
E. faecium (2018, Female, <1 y) HUG |
256 (R) | >64 (R) | R | + | 12 (R) | 2 (S) | 0.75 (S) |
|
E. faecium (2018, Female, <1 y) HUG |
>256 (R) | >64 (R) | R | + | 24 (R) | 3 (S) | 1 (S) |
|
E. faecium (2018, Male, 30 y) HUG |
32 (R) | 64 (R) | R | + | 1 (S) | 2 (S) | 1 (S) |
|
E. faecium (2018, Male, <1 y) HUG |
256 (R) | >64 (R) | R | + | 16 (R) | 3 (S) | 1 (S) |
|
E. faecium (2018, Male, 58 y) HUG |
>256 (R) | >64 (R) | R | + | 96 (R) | 4 (S) | 0.5 (S) |
|
E. faecium (2018, Male, 84 y) HUG |
128 (R) | >64 (R) | R | + | 1 (S) | 2 (S) | 0.75 (S) |
|
E. faecium (2018, Male, 90 y) HUG |
48 (R) | >64 (R) | R | + | 0.75 (S) | 2 (S) | 0.75 (S) |
|
E. faecium (2018, Female, 7 y) HUG |
>256 (R) | >64 (R) | R | + | 64 (R) | 8 (R) | 0.75 (S) |
VAN, Vancomycin; MIC, Minimum Inhibitory Concentration.
*
Fuzzy zone edges,
**
Presence of a resistant heteropopulation.
R, resistant; S, susceptible.
Daptomycin MICs were interpreted according to CLSI breakpoints.