TABLE 2.
Mutant phenotypes
| Strain | Time of agar invasion (days)a |
wt lossb | Fungal burdenc |
Hyphae in tongue histologyd |
Hyphae in tongue homogenated |
|---|---|---|---|---|---|
| Wild type | 2 | ++++ | ++++ | ++++ | ++++ |
| orf19.4880Δ | 4 | ++++ | ++++ | +++ | +++ |
| orf19.716Δ | 4 | ++++ | ++++ | ++ | + |
| spe1Δ | >7 | ++++ | + | − | +/− |
| spf1Δ | 4 | ++++ | ++++ | ++ | ++ |
| orf19.2336Δ | 4 | ++++ | +++ | ++ | ++ |
| kre5Δ | >4 | ++ | +++ | + | ++ |
| orf19.3751Δ | >4 | ++ | ++++ | − | +/− |
| rvs161Δ | ND | + | +/− | − | − |
| rvs167Δ | ND | + | +/− | − | − |
Days of incubation after which the strains started to show detectable invasion into agar containing serum or GlcNAc. > indicates that invasion was seen for only one of the stimuli. Note that none of the mutants showed significant invasive growth in agar medium at alkaline pH or in spider medium after 7 days, whereas the wild-type control showed invasion after 2 days. ND, not determined.
++++, not significantly different; ++, about one-half of the weight loss; +, about one-third of the weight loss seen for mice infected with wild-type control C. albicans.
Measured as median CFU per gram of tongue. ++++, ≤2-fold lower; +++, ≤5-fold lower; +, <8-fold lower; +/−, ∼10-fold lower, with some mice lacking detectable CFU.
Presence of hyphae. ++++, nearly all hyphae; ++, presence of both hyphae and buds; +, rare hyphae and mostly buds; −, no or rarely detectable hyphae.