FIG 5.

QKO BMMs that additionally lack CASP11, IRG1, or both factors display partial to complete lack of restriction of L. pneumophila replication when stimulated with IFN-γ. (A) Log₁₀ RLU from LP02 ΔflaA lux L. pneumophila from infected wild-type C57BL/6, QKO, QKO/C11, QKO/IRG1, and 6KO BMMs either not stimulated (no stim) or stimulated with 6.0 ng/ml IFN-γ (IFN-γ). ***, P < 0.001; ns, not significant, comparing indicated curves by 2-way ANOVA. (B) Log₁₀ CFU recovered from WT C57BL/6, QKO, QKO/C11, QKO/IRG1, and 6KO BMMs 48 h following infection with LP02 ΔflaA lux L. pneumophila. ***, P < 0.001; *, P < 0.05; ns, not significant, comparing means by unpaired t test. Data in panels A and B are pooled from multiple independent experiments using BMMs derived from two or more different mice per genotype. Error bars in panels A and B represent standard deviation of the mean as follows: C57BL/6, RLU, n = 5, and CFU, n = 5 independent experiments; QKO, RLU, n = 9, and CFU, n = 3 independent experiments; QKO/C11, RLU, n = 4, and CFU, n = 3 independent experiments; QKO/IRG1, RLU, n = 8, and CFU, n = 3 independent experiments; 6KO, RLU, n = 5, and CFU, n = 3 independent experiments. (C) Log₁₀ RLU from LP02 ΔflaA ΔuhpC lux L. pneumophila from infected QKO, Nos2^−/− Acod1^−/−, and QKO/IRG1 BMMs stimulated with 6.0 ng/ml IFN-γ and 2.0 mM 2DG as indicated. ***, P < 0.001; ns, not significant, comparing indicated curves by 2-way ANOVA. Data in panel C reflect results of individual experiments that represent at least two independent experiments.