TABLE 1.
Guide RNAs used in this study for CRISPR
| Guide RNA target | gRNA sequence | PAMb | Genotype(s) to which contributing |
|---|---|---|---|
| iCas9 BMMsa | |||
| Ifngr1 | GGTATTCCCAGCATACGACA | GGG | iCas9::Ifngr1 |
| Irg1 (Acod1) exon 2 | GGACAGATGGTATCATTCGG | AGG | iCas9::Acod1, iCas9::Nos2Acod1 |
| Irg1 (Acod1) exon 3 | GAAAAGCAGCATATGTCGGT | GGG | iCas9::Acod1, iCas9::Nos2Acod1 |
| Nos2 exon 2 | GTCTTTCAGGTCACTTTGGT | AGG | iCas9::Nos2, iCas9::Nos2Acod1 |
| Nos2 intron 2-3 | GTCAGTAGTGACGTCCTGAT | TGG | iCas9::Nos2, iCas9::Nos2Acod1 |
| QKO mouse embryos | |||
| Irg1 (Acod1) exon 2 | TGACAGATGGTATCATTCGG | AGG | QKO/IRG1, 6KO |
| Irg1 (Acod1) exon 3 | CAAAAGCAGCATATGTCGGT | GGG | QKO/IRG1, 6KO |
| Caspase-11 (Casp4) exon 5 | GTATCATACTGTAGCACATC | TGG | QKO/C11, 6KO |
| Caspase-11 (Casp4) intron 4-5 | ATGTTGATTTTACCGAAATG | AGG | QKO/C11, 6KO |
a
Requires gRNA sequence prepended with G.
b
PAM, protospacer-adjacent motif.