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. 2019 Sep 27;294(45):17060–17074. doi: 10.1074/jbc.RA119.010835

Figure 2.

Figure 2.

Expression profile of Rdhe2 and Rdhe2s transcripts in mouse tissues. A, qPCR analysis of Rdhe2 and Rdhe2s expression in adult tissues of two animals. Inset, PCR-amplified full-length Rdhe2 and Rdhe2s transcripts. Lanes 1 and 2, tongue; lanes 3 and 4, esophagus; lanes 5 and 6, stomach; lanes 7 and 8, skin; lane 9, adipose tissue; lanes 10 and 11, intestine; lanes 12 and 13, brain; lanes 14 and 15, testis; lanes 16 and 17, heart; lanes 18 and 19, lung; lanes 20 and 21, liver; lanes 22 and 23, kidney; lanes 24 and 25, eyes; lanes 26 and 27, colon. B, semiquantitative PCR analysis of temporal expression patterns of Rdhe2 and Rdhe2s transcript fragments in embryonic mouse tissues. C57BL/J6 embryos were collected at E9.5–E14.5 stages of development as indicated. Five μg of mRNA was used for reverse transcription with random hexamer primers, and one-twentieth of the resulting cDNA was used per reaction. Gapdh was used as a loading control.