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. 2019 Sep 27;294(45):17060–17074. doi: 10.1074/jbc.RA119.010835

Figure 7.

Figure 7.

Eye phenotype of DKO mice. A, histology of whole eye lids. Magnification, ×60. Note the longer and thicker appearance of lower and upper eyelids from DKO mice. Four biological replicas were analyzed from littermates WT#38/DKO1#37, WT#39/DKO2#40, WT#41/DKO2#38 and WT#18/DKO1#20. B, immunohistology of eyelid sections using custom-made RDHE2S antibodies at a 1:400 dilution. C, immunohistology of eyelid sections using commercial SDR16C5 (RDHE2) antibodies (Sigma αSDR16C5 #HPA 025224, lot R11757) at a 1:200 dilution. Both RDHE2S and RDHE2 are localized in meibomian glands and sebaceous glands in eyelids. As determined by measuring a representative image of eyelid sections using the image-processing program ImageJ (33), the DKO meibomian glands are nearly twice the size (198%) of the WT meibomian glands. Overall, the eyelids of DKO mice are 122.5 ± 1.3% the size of WT eyelids (n = 3 pairs of littermates). Scale bar, 200 μm, 100-fold magnification.