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. 2019 Sep 22;294(45):16740–16755. doi: 10.1074/jbc.RA119.009992

Figure 4.

Figure 4.

CDS2 deficiency promoted LD-association of DGAT2. A, localization of DGAT1 in CDS KD cells after incubation with 200 μm OA for 16 h. Bars, 5 μm. B, quantification of LD sizes in cells transfected by GFP-DGAT1 upon CDS deficiency. Two-tailed Student's t test was used: mean; n = 150–300 LDs from ∼20 cells; ***, p < 0.001; ****, p < 0.0001. C, distribution of LDs in cells transfected by GFP-DGAT1 upon CDS deficiency. LDs from ∼20 cells/cell type were used. D, localization of DGAT2 in CDS KD cells after incubation with 200 μm OA for 16 h. Bars, 5 μm. E, quantification of LD sizes in cells transfected by GFP-DGAT2 upon CDS deficiency. Two-tailed Student's t test was used: mean; n = ∼1000 LDs for siCtrl and siCDS1 and 300 LDs for siCDS2; ****, p < 0.0001; ns, no significance. F, distribution of LDs in cells transfected by GFP-DGAT2 upon CDS deficiency. LDs from ∼20 cells/cell type were used. G, localization of DGAT2 at the early stage of LD formation in CDS KD cells. 200 μm oleate was added to cells for the indicated time. Bars, 5 μm. White arrows, DGAT2 puncta colocalizing with LDs. H, quantification of DGAT2-LD associations during LD growth upon CDS deficiency. Two-tailed Student's t test was used: mean ± S.D. (error bars); n = 20 cells; *, p < 0.05; ns, no significance.