Figure 2.

Three variants of human αENaC changed channel activity in Xenopus oocytes. Oocytes expressing WT and mutant human ENaCs were clamped at −100 mV (membrane potential), and whole-cell currents were recorded in a bath solution (NaCl-110, containing 110 mm NaCl) in the absence and presence of 10 μm amiloride. A, C, E, and G, representative recordings of WT and mutant ENaCs. Traces were superimposed with the same time and current scales. Negative values reflect inward Na⁺ currents. B, D, F, and H, normalized currents, representing amiloride-sensitive currents in all cells that were divided by the mean of the WT group in the same batch of oocytes. Data were pooled from three to five batches of oocytes. Dot plots were overlaid with mean ± S.D. The p values were from Student's t test.